Runs BLASTp of a single peptide FASTA file vs phylogenetic subsets of Uniprot accessions provided by AgBase. Transfers gene ontology (GO) annotations to from BLASTp matches to the query gene products.
- GOanna accepts a single peptide FASTA file.
- Resources: https://agbase.arizona.edu/cgi-bin/tools/GOanna.cgi, https://github.com/AgBase/GOanna, https://agbase-docs.readthedocs.io/en/latest/
Test data for this app appears directly in the Discovery Environment in the Data window under Community Data -> iplantcollaborative -> example_data -> GOanna
Use CFLO_1.fa from the directory above as test input.
Parameters Used in App
Use the Default parameters with the Test data. Fill in the output section like so:
Output file basename: clfo1_vs_insecta
Database of query id: i5k
Assigned by field for your GAF output file: <your name>
Taxon ID of the peptide you are BLASTing: 29053
clfo1_v_insecta.asn–BLAST output for conversion to other formats
clfo1_v_insecta.html–pairwise alignments from BLAST search
clfo1_v_insecta.tsv--tab-delimited BLAST output
clfo1_v_insecta_goanna_gaf.tsv–gene association file with transferred GO annotations