This box searches only this space. The box at the upper right searches the entire iPlant wiki.

Skip to end of metadata
Go to start of metadata

Organizers:

Molly Cohen, ADE

Local Point of Contact:

Marni Landry, PVHS

Trainers:

Uwe Hilgert, UA/BIO5 Institute

Date:

February 11, 2017

Location:

NW corner of 40th St. and Bell, Phoenix, Paradise Valley

Requirements

 

Make sure you have a valid CyVerse/iPlant account:

 

Workshop Agenda and Resources

Time

Topic

Slides

Notes/Links

8:00-12:00

DNA Barcoding - Isolate DNA & Prep PCR

 

DNA Barcoding Protocol

12:00-01:00

Lunch

 

 

01:00-02:30

DNA Barcoding - Electrophoresis and Prep DNA for Sequencing

 

Barcoding lab prep and planning

02:30-05:00

Analyze DNA Barcodes (Blue Line)

 

Blue Line Walk-through

Links to updated protocol, list of barcoding samples and gel images

 Updated protocol used in workshop (Word doc will allow you to edit doc to your own circumstances)

List of  Sample numbers / Experimentor names / Presumed name of plant / School

Gel Images for Samples #1-#9 and Samples #10-#17.

Ms. Cherry's movie of the gel that holds Samples #1-#9.

Post Workshop DNA Sequencing Results

Instructions on retrieving your results will be posted here after the workshop.

DNA Subway instructions:

Log into DNA Subway (dnasubway.cyverse.org)

Start a blue line project

  1. Under 'Select Project Type' select the project type to be ‘rbcL’ barcoding
  2. Under ‘Select Sequence Source’ choose ‘Import Sequence from DNALC.’ Our sequences have a the Tracking #: 10-363917407 and are posted as the entry under Date: 02/25/2017 and Name: Uwe Hilgert.
  3. Check 'select all,' then click on 'Add Selected Files.'
  4. Provide a 'Project title.'
  5. Provide a project 'Description.'
  6. Click on 'Continue.'

Conduct the analysis following the instructions in Blue Line Walk-through.

Tip: Process at first only your own sample through all the steps. Then, as you become more familiar with DNA Subway and sequence analysis, look at some of the other sample.

 

Additional Links and Resources

 

  • No labels