Summary of Fastqc Reports


Fastq File Name Basic Statistics Per base sequence quality Per sequence quality scores Per base sequence content Per base GC content Per sequence GC content Per base N content Sequence Length Distribution Sequence Duplication Levels Overrepresented sequences Kmer Content
TrmPr1_SRR566981.sra_1.fastq
  • [PASS] Basic Statistics
    		•
  • [PASS] Per base sequence quality
    		•
  • [PASS] Per sequence quality scores
    		•
  • [PASS] Per base sequence content
    		•
  • [PASS] Per base GC content
    		•
  • [WARNING] Per sequence GC content
    		•
  • [PASS] Per base N content
    		•
  • [WARNING] Sequence Length Distribution
    		•
  • [WARNING] Sequence Duplication Levels
    		•
  • [PASS] Overrepresented sequences
    		•
  • [PASS] Kmer Content
    		•
    TrmPr1_SRR567164.sra_1.fastq
  • [PASS] Basic Statistics
    		•
  • [PASS] Per base sequence quality
    		•
  • [PASS] Per sequence quality scores
    		•
  • [PASS] Per base sequence content
    		•
  • [PASS] Per base GC content
    		•
  • [WARNING] Per sequence GC content
    		•
  • [PASS] Per base N content
    		•
  • [WARNING] Sequence Length Distribution
    		•
  • [FAIL] Sequence Duplication Levels
    		•
  • [PASS] Overrepresented sequences
    		•
  • [PASS] Kmer Content
    		•
    TrmPr1_SRR567165.sra_1.fastq
  • [PASS] Basic Statistics
    		•
  • [PASS] Per base sequence quality
    		•
  • [PASS] Per sequence quality scores
    		•
  • [PASS] Per base sequence content
    		•
  • [PASS] Per base GC content
    		•
  • [WARNING] Per sequence GC content
    		•
  • [PASS] Per base N content
    		•
  • [WARNING] Sequence Length Distribution
    		•
  • [WARNING] Sequence Duplication Levels
    		•
  • [PASS] Overrepresented sequences
    		•
  • [PASS] Kmer Content
    		•
    TrmPr2_SRR566981.sra_2.fastq
  • [PASS] Basic Statistics
    		•
  • [PASS] Per base sequence quality
    		•
  • [PASS] Per sequence quality scores
    		•
  • [PASS] Per base sequence content
    		•
  • [PASS] Per base GC content
    		•
  • [WARNING] Per sequence GC content
    		•
  • [PASS] Per base N content
    		•
  • [WARNING] Sequence Length Distribution
    		•
  • [WARNING] Sequence Duplication Levels
    		•
  • [PASS] Overrepresented sequences
    		•
  • [PASS] Kmer Content
    		•
    TrmPr2_SRR567164.sra_2.fastq
  • [PASS] Basic Statistics
    		•
  • [PASS] Per base sequence quality
    		•
  • [PASS] Per sequence quality scores
    		•
  • [PASS] Per base sequence content
    		•
  • [PASS] Per base GC content
    		•
  • [WARNING] Per sequence GC content
    		•
  • [PASS] Per base N content
    		•
  • [WARNING] Sequence Length Distribution
    		•
  • [FAIL] Sequence Duplication Levels
    		•
  • [PASS] Overrepresented sequences
    		•
  • [PASS] Kmer Content
    		•
    TrmPr2_SRR567165.sra_2.fastq
  • [PASS] Basic Statistics
    		•
  • [PASS] Per base sequence quality
    		•
  • [PASS] Per sequence quality scores
    		•
  • [PASS] Per base sequence content
    		•
  • [PASS] Per base GC content
    		•
  • [WARNING] Per sequence GC content
    		•
  • [PASS] Per base N content
    		•
  • [WARNING] Sequence Length Distribution
    		•
  • [WARNING] Sequence Duplication Levels
    		•
  • [PASS] Overrepresented sequences
    		•
  • [PASS] Kmer Content
    		•
    TrmS_Belgica_control.fastq
  • [PASS] Basic Statistics
    		•
  • [PASS] Per base sequence quality
    		•
  • [PASS] Per sequence quality scores
    		•
  • [PASS] Per base sequence content
    		•
  • [PASS] Per base GC content
    		•
  • [WARNING] Per sequence GC content
    		•
  • [PASS] Per base N content
    		•
  • [WARNING] Sequence Length Distribution
    		•
  • [WARNING] Sequence Duplication Levels
    		•
  • [PASS] Overrepresented sequences
    		•
  • [WARNING] Kmer Content
    		•
    TrmSos_SRR566981.sra_1.fastq
  • [PASS] Basic Statistics
    		•
  • [PASS] Per base sequence quality
    		•
  • [PASS] Per sequence quality scores
    		•
  • [PASS] Per base sequence content
    		•
  • [PASS] Per base GC content
    		•
  • [WARNING] Per sequence GC content
    		•
  • [PASS] Per base N content
    		•
  • [WARNING] Sequence Length Distribution
    		•
  • [PASS] Sequence Duplication Levels
    		•
  • [PASS] Overrepresented sequences
    		•
  • [WARNING] Kmer Content
    		•
    TrmSos_SRR567164.sra_1.fastq
  • [PASS] Basic Statistics
    		•
  • [PASS] Per base sequence quality
    		•
  • [PASS] Per sequence quality scores
    		•
  • [PASS] Per base sequence content
    		•
  • [PASS] Per base GC content
    		•
  • [WARNING] Per sequence GC content
    		•
  • [PASS] Per base N content
    		•
  • [WARNING] Sequence Length Distribution
    		•
  • [WARNING] Sequence Duplication Levels
    		•
  • [PASS] Overrepresented sequences
    		•
  • [WARNING] Kmer Content
    		•
    TrmSos_SRR567165.sra_1.fastq
  • [PASS] Basic Statistics
    		•
  • [PASS] Per base sequence quality
    		•
  • [PASS] Per sequence quality scores
    		•
  • [PASS] Per base sequence content
    		•
  • [PASS] Per base GC content
    		•
  • [WARNING] Per sequence GC content
    		•
  • [PASS] Per base N content
    		•
  • [WARNING] Sequence Length Distribution
    		•
  • [PASS] Sequence Duplication Levels
    		•
  • [WARNING] Overrepresented sequences
    		•
  • [WARNING] Kmer Content
    		•




    Basic Statistics Per base sequence quality Per sequence quality scores Per base sequence content Per base GC content Per sequence GC content Per base N content Sequence Length Distribution Sequence Duplication Levels Overrepresented sequences Kmer Content

    TrmPr1_SRR566981.sra_1.fastq

    [OK] Basic Statistics

    Measure Value
    Filename TrmPr1_SRR566981.sra_1.fastq
    File type Conventional base calls
    Encoding Illumina 1.5
    Total Sequences 1336440
    Filtered Sequences 0
    Sequence length 45-66
    %GC 46

    TrmPr1_SRR566981.sra_1.fastq

    [OK] Per base sequence quality

    Per base quality graph

    TrmPr1_SRR566981.sra_1.fastq

    [OK] Per sequence quality scores

    Per Sequence quality graph

    TrmPr1_SRR566981.sra_1.fastq

    [OK] Per base sequence content

    Per base sequence content

    TrmPr1_SRR566981.sra_1.fastq

    [OK] Per base GC content

    Per base GC content graph

    TrmPr1_SRR566981.sra_1.fastq

    [WARN] Per sequence GC content

    Per sequence GC content graph

    TrmPr1_SRR566981.sra_1.fastq

    [OK] Per base N content

    N content graph

    TrmPr1_SRR566981.sra_1.fastq

    [WARN] Sequence Length Distribution

    Sequence length distribution

    TrmPr1_SRR566981.sra_1.fastq

    [WARN] Sequence Duplication Levels

    Duplication level graph

    TrmPr1_SRR566981.sra_1.fastq

    [OK] Overrepresented sequences

    No overrepresented sequences

    TrmPr1_SRR566981.sra_1.fastq

    [OK] Kmer Content

    "

    TrmPr1_SRR567164.sra_1.fastq

    [OK] Basic Statistics

    Measure Value
    Filename TrmPr1_SRR567164.sra_1.fastq
    File type Conventional base calls
    Encoding Illumina 1.5
    Total Sequences 11245742
    Filtered Sequences 0
    Sequence length 45-64
    %GC 46

    TrmPr1_SRR567164.sra_1.fastq

    [OK] Per base sequence quality

    Per base quality graph

    TrmPr1_SRR567164.sra_1.fastq

    [OK] Per sequence quality scores

    Per Sequence quality graph

    TrmPr1_SRR567164.sra_1.fastq

    [OK] Per base sequence content

    Per base sequence content

    TrmPr1_SRR567164.sra_1.fastq

    [OK] Per base GC content

    Per base GC content graph

    TrmPr1_SRR567164.sra_1.fastq

    [WARN] Per sequence GC content

    Per sequence GC content graph

    TrmPr1_SRR567164.sra_1.fastq

    [OK] Per base N content

    N content graph

    TrmPr1_SRR567164.sra_1.fastq

    [WARN] Sequence Length Distribution

    Sequence length distribution

    TrmPr1_SRR567164.sra_1.fastq

    [FAIL] Sequence Duplication Levels

    Duplication level graph

    TrmPr1_SRR567164.sra_1.fastq

    [OK] Overrepresented sequences

    No overrepresented sequences

    TrmPr1_SRR567164.sra_1.fastq

    [OK] Kmer Content

    "

    TrmPr1_SRR567165.sra_1.fastq

    [OK] Basic Statistics

    Measure Value
    Filename TrmPr1_SRR567165.sra_1.fastq
    File type Conventional base calls
    Encoding Illumina 1.5
    Total Sequences 1119862
    Filtered Sequences 0
    Sequence length 45-66
    %GC 46

    TrmPr1_SRR567165.sra_1.fastq

    [OK] Per base sequence quality

    Per base quality graph

    TrmPr1_SRR567165.sra_1.fastq

    [OK] Per sequence quality scores

    Per Sequence quality graph

    TrmPr1_SRR567165.sra_1.fastq

    [OK] Per base sequence content

    Per base sequence content

    TrmPr1_SRR567165.sra_1.fastq

    [OK] Per base GC content

    Per base GC content graph

    TrmPr1_SRR567165.sra_1.fastq

    [WARN] Per sequence GC content

    Per sequence GC content graph

    TrmPr1_SRR567165.sra_1.fastq

    [OK] Per base N content

    N content graph

    TrmPr1_SRR567165.sra_1.fastq

    [WARN] Sequence Length Distribution

    Sequence length distribution

    TrmPr1_SRR567165.sra_1.fastq

    [WARN] Sequence Duplication Levels

    Duplication level graph

    TrmPr1_SRR567165.sra_1.fastq

    [OK] Overrepresented sequences

    No overrepresented sequences

    TrmPr1_SRR567165.sra_1.fastq

    [OK] Kmer Content

    "

    TrmPr2_SRR566981.sra_2.fastq

    [OK] Basic Statistics

    Measure Value
    Filename TrmPr2_SRR566981.sra_2.fastq
    File type Conventional base calls
    Encoding Illumina 1.5
    Total Sequences 1336440
    Filtered Sequences 0
    Sequence length 45-66
    %GC 46

    TrmPr2_SRR566981.sra_2.fastq

    [OK] Per base sequence quality

    Per base quality graph

    TrmPr2_SRR566981.sra_2.fastq

    [OK] Per sequence quality scores

    Per Sequence quality graph

    TrmPr2_SRR566981.sra_2.fastq

    [OK] Per base sequence content

    Per base sequence content

    TrmPr2_SRR566981.sra_2.fastq

    [OK] Per base GC content

    Per base GC content graph

    TrmPr2_SRR566981.sra_2.fastq

    [WARN] Per sequence GC content

    Per sequence GC content graph

    TrmPr2_SRR566981.sra_2.fastq

    [OK] Per base N content

    N content graph

    TrmPr2_SRR566981.sra_2.fastq

    [WARN] Sequence Length Distribution

    Sequence length distribution

    TrmPr2_SRR566981.sra_2.fastq

    [WARN] Sequence Duplication Levels

    Duplication level graph

    TrmPr2_SRR566981.sra_2.fastq

    [OK] Overrepresented sequences

    No overrepresented sequences

    TrmPr2_SRR566981.sra_2.fastq

    [OK] Kmer Content

    "

    TrmPr2_SRR567164.sra_2.fastq

    [OK] Basic Statistics

    Measure Value
    Filename TrmPr2_SRR567164.sra_2.fastq
    File type Conventional base calls
    Encoding Illumina 1.5
    Total Sequences 11245742
    Filtered Sequences 0
    Sequence length 45-64
    %GC 46

    TrmPr2_SRR567164.sra_2.fastq

    [OK] Per base sequence quality

    Per base quality graph

    TrmPr2_SRR567164.sra_2.fastq

    [OK] Per sequence quality scores

    Per Sequence quality graph

    TrmPr2_SRR567164.sra_2.fastq

    [OK] Per base sequence content

    Per base sequence content

    TrmPr2_SRR567164.sra_2.fastq

    [OK] Per base GC content

    Per base GC content graph

    TrmPr2_SRR567164.sra_2.fastq

    [WARN] Per sequence GC content

    Per sequence GC content graph

    TrmPr2_SRR567164.sra_2.fastq

    [OK] Per base N content

    N content graph

    TrmPr2_SRR567164.sra_2.fastq

    [WARN] Sequence Length Distribution

    Sequence length distribution

    TrmPr2_SRR567164.sra_2.fastq

    [FAIL] Sequence Duplication Levels

    Duplication level graph

    TrmPr2_SRR567164.sra_2.fastq

    [OK] Overrepresented sequences

    No overrepresented sequences

    TrmPr2_SRR567164.sra_2.fastq

    [OK] Kmer Content

    "

    TrmPr2_SRR567165.sra_2.fastq

    [OK] Basic Statistics

    Measure Value
    Filename TrmPr2_SRR567165.sra_2.fastq
    File type Conventional base calls
    Encoding Illumina 1.5
    Total Sequences 1119862
    Filtered Sequences 0
    Sequence length 45-66
    %GC 46

    TrmPr2_SRR567165.sra_2.fastq

    [OK] Per base sequence quality

    Per base quality graph

    TrmPr2_SRR567165.sra_2.fastq

    [OK] Per sequence quality scores

    Per Sequence quality graph

    TrmPr2_SRR567165.sra_2.fastq

    [OK] Per base sequence content

    Per base sequence content

    TrmPr2_SRR567165.sra_2.fastq

    [OK] Per base GC content

    Per base GC content graph

    TrmPr2_SRR567165.sra_2.fastq

    [WARN] Per sequence GC content

    Per sequence GC content graph

    TrmPr2_SRR567165.sra_2.fastq

    [OK] Per base N content

    N content graph

    TrmPr2_SRR567165.sra_2.fastq

    [WARN] Sequence Length Distribution

    Sequence length distribution

    TrmPr2_SRR567165.sra_2.fastq

    [WARN] Sequence Duplication Levels

    Duplication level graph

    TrmPr2_SRR567165.sra_2.fastq

    [OK] Overrepresented sequences

    No overrepresented sequences

    TrmPr2_SRR567165.sra_2.fastq

    [OK] Kmer Content

    "

    TrmS_Belgica_control.fastq

    [OK] Basic Statistics

    Measure Value
    Filename TrmS_Belgica_control.fastq
    File type Conventional base calls
    Encoding Illumina 1.5
    Total Sequences 526438
    Filtered Sequences 0
    Sequence length 45-66
    %GC 47

    TrmS_Belgica_control.fastq

    [OK] Per base sequence quality

    Per base quality graph

    TrmS_Belgica_control.fastq

    [OK] Per sequence quality scores

    Per Sequence quality graph

    TrmS_Belgica_control.fastq

    [OK] Per base sequence content

    Per base sequence content

    TrmS_Belgica_control.fastq

    [OK] Per base GC content

    Per base GC content graph

    TrmS_Belgica_control.fastq

    [WARN] Per sequence GC content

    Per sequence GC content graph

    TrmS_Belgica_control.fastq

    [OK] Per base N content

    N content graph

    TrmS_Belgica_control.fastq

    [WARN] Sequence Length Distribution

    Sequence length distribution

    TrmS_Belgica_control.fastq

    [WARN] Sequence Duplication Levels

    Duplication level graph

    TrmS_Belgica_control.fastq

    [OK] Overrepresented sequences

    No overrepresented sequences

    TrmS_Belgica_control.fastq

    [WARN] Kmer Content

    TrmSos_SRR566981.sra_1.fastq

    [OK] Basic Statistics

    Measure Value
    Filename TrmSos_SRR566981.sra_1.fastq
    File type Conventional base calls
    Encoding Illumina 1.5
    Total Sequences 75362
    Filtered Sequences 0
    Sequence length 45-66
    %GC 47

    TrmSos_SRR566981.sra_1.fastq

    [OK] Per base sequence quality

    Per base quality graph

    TrmSos_SRR566981.sra_1.fastq

    [OK] Per sequence quality scores

    Per Sequence quality graph

    TrmSos_SRR566981.sra_1.fastq

    [OK] Per base sequence content

    Per base sequence content

    TrmSos_SRR566981.sra_1.fastq

    [OK] Per base GC content

    Per base GC content graph

    TrmSos_SRR566981.sra_1.fastq

    [WARN] Per sequence GC content

    Per sequence GC content graph

    TrmSos_SRR566981.sra_1.fastq

    [OK] Per base N content

    N content graph

    TrmSos_SRR566981.sra_1.fastq

    [WARN] Sequence Length Distribution

    Sequence length distribution

    TrmSos_SRR566981.sra_1.fastq

    [OK] Sequence Duplication Levels

    Duplication level graph

    TrmSos_SRR566981.sra_1.fastq

    [OK] Overrepresented sequences

    No overrepresented sequences

    TrmSos_SRR566981.sra_1.fastq

    [WARN] Kmer Content

    TrmSos_SRR567164.sra_1.fastq

    [OK] Basic Statistics

    Measure Value
    Filename TrmSos_SRR567164.sra_1.fastq
    File type Conventional base calls
    Encoding Illumina 1.5
    Total Sequences 399575
    Filtered Sequences 0
    Sequence length 45-64
    %GC 47

    TrmSos_SRR567164.sra_1.fastq

    [OK] Per base sequence quality

    Per base quality graph

    TrmSos_SRR567164.sra_1.fastq

    [OK] Per sequence quality scores

    Per Sequence quality graph

    TrmSos_SRR567164.sra_1.fastq

    [OK] Per base sequence content

    Per base sequence content

    TrmSos_SRR567164.sra_1.fastq

    [OK] Per base GC content

    Per base GC content graph

    TrmSos_SRR567164.sra_1.fastq

    [WARN] Per sequence GC content

    Per sequence GC content graph

    TrmSos_SRR567164.sra_1.fastq

    [OK] Per base N content

    N content graph

    TrmSos_SRR567164.sra_1.fastq

    [WARN] Sequence Length Distribution

    Sequence length distribution

    TrmSos_SRR567164.sra_1.fastq

    [WARN] Sequence Duplication Levels

    Duplication level graph

    TrmSos_SRR567164.sra_1.fastq

    [OK] Overrepresented sequences

    No overrepresented sequences

    TrmSos_SRR567164.sra_1.fastq

    [WARN] Kmer Content

    TrmSos_SRR567165.sra_1.fastq

    [OK] Basic Statistics

    Measure Value
    Filename TrmSos_SRR567165.sra_1.fastq
    File type Conventional base calls
    Encoding Illumina 1.5
    Total Sequences 51501
    Filtered Sequences 0
    Sequence length 45-66
    %GC 47

    TrmSos_SRR567165.sra_1.fastq

    [OK] Per base sequence quality

    Per base quality graph

    TrmSos_SRR567165.sra_1.fastq

    [OK] Per sequence quality scores

    Per Sequence quality graph

    TrmSos_SRR567165.sra_1.fastq

    [OK] Per base sequence content

    Per base sequence content

    TrmSos_SRR567165.sra_1.fastq

    [OK] Per base GC content

    Per base GC content graph

    TrmSos_SRR567165.sra_1.fastq

    [WARN] Per sequence GC content

    Per sequence GC content graph

    TrmSos_SRR567165.sra_1.fastq

    [OK] Per base N content

    N content graph

    TrmSos_SRR567165.sra_1.fastq

    [WARN] Sequence Length Distribution

    Sequence length distribution

    TrmSos_SRR567165.sra_1.fastq

    [OK] Sequence Duplication Levels

    Duplication level graph

    TrmSos_SRR567165.sra_1.fastq

    [WARN] Overrepresented sequences

    Sequence Count Percentage Possible Source
    CAACGAATGCTTCGTAGAAGAAGACGATACGCTTGTAGAAGACATAAAACATCGGATCACGCAGGG 67 0.13009456127065494 No Hit

    TrmSos_SRR567165.sra_1.fastq

    [WARN] Kmer Content