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- To use Picard Preprocess, import your alignment output data in SAM/BAM format.
- *Resources: http://broadinstitute.github.io/picard/index.html
Test data for this app appears directly in the Discovery Environment in the Data window under Shared With Me” -> luj -> variant_calling.
Use directory bwa_output from the directory above as test input directory containing alignment output.
Parameters Used in App
When the app is run in the Discovery Environment, use the following parameters with the above input directory to get the output provided in the next section below.
- Default parameters only, no further configuration needed.
The app will extract read group info from the file names if you following assumed naming convention as following:
the read group id is 120319_I631_FCC0J86ACXX_L4
the read group sample name is CAAS_B093
the read group platform unit is 120319_I631
the read group platform is Illumina which is default
the read group library is RICxaoRSYHSD8-4-IPAAPEKF-62
All except read group platform will be extracted from file name if you leave the read group info field empty in Parameters panel. If your file naming convention is different, you have to enter one bam file as input a time and enter the read group information in the Parameters panel.
Expect a directory file named picard_preprocess_output as output directory containing processed alignment outputs.