This box searches only this space. The box at the upper right searches the entire iPlant wiki.

Versions Compared

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.

...


One quick observation is that very short 1kP scaffolds tend to be classified as bad, but the fraction of good scaffolds improves with greater length. (see TransratePassRate.png)  Scaffolds of 300 bp or longer, are overwhelmingly (89%) rated as good.  While we previously recommended that sub-300 bp scaffolds be ignored for most analyses, you may be interested in this alternative argument for doing so.

Steve Steven Kelly cautions that the automated scaffold cut-off is great for rejecting bad assemblies, but it does discard some correct assemblies.  He suggests that for OneKP it should be generally fine, as most of SOAPdenovo-Trans errors result from multiple fragments of larger scaffolds already present in the assembly.  A very conservative alternative cut off is to just throw away anything with a minimum score, i.e. 0.01, indicating the scaffold has no supporting reads.

...