Please work through the documentation and add your comments on the bottom of this page, or email comments to email@example.com. Thank you.
Rationale and Background
The makeblastdb application produces BLAST databases from FASTA files. In the simplest case the FASTA definition lines are not parsed by makeblastdb and may be completely unstructured. The text in the definition line will be stored in the BLAST database and displayed in the BLAST report
- Leave these as default
- With plant.118.1.genomic.fna as input file and nucleotide as sequence format
- With plant.118.protein.faa as input file and protein as sequence format
The Blastp-2.6.0+ and Blastn-2.6.0+ apps take a folder as input for the database because there are multiple files involved. The best thing to do when you are creating a database is to give the database and the output file the same name e.g. "mygenome". Then after it has run, make a new folder inside the output directory, name it "mygenome", and drag all the database files into it, but not the logs directory. You can then drag that directory "mygenome" to one of your other directories so it will be easy to find. When you run Blastp or Blastn drag and drop the database directory you created into the database input for Blastp/Blastn
Please work through the documentation and add your comments on the bottom of this page, or email comments to firstname.lastname@example.org or click the intercom button on this page. Thank you.
For more options of makeblastdb visit this page