Genomics in Education Workshop at Prairie View A&M

Organizers:

Jason Williams

Local Point of Contact:

Gloria Regisford

Trainers:

Jason Williams, John Fonner

Date:

Sept 26-27, 2013

Location:

Prairie View Texas A&M University
FM 1098 Rd & University Dr.
Prairie View, TX 77446
936-261-3311
E.E. O’Banion Building, Room Number 123
Wet Lab: E.E. O’Banion Building, Room Number 406

Section Navigation

 Pre-Workshop Homework

Laptop Compatibility

If you are bringing your own laptop:

Most laptops (<5 years old) updated with the latest web browser (we recommend FireFox:http://www.mozilla.org/en-US/firefox/fx/ ) will have no problem.

Here are some things to check:

  • If possible, make sure you have have administrative privileges on your computer (should it become necessary to install or update software during the workshop).
  • Ensure that your computer is running a current browser (Mozilla Firefox 13/ Internet Explorer 8 (IE 9 preferred) / Safari 4 or later).
  • Be aware, Java 7 has known issues that limit some functionality on DNA Subway. Get Java 6 @ this link: http://java.com/en/download/manual_v6.jsp
  • If you are a Windows user, ensure that your computer is running Java 6 Update 19 or later by going to http://java.com and click ‘Do I Have Java?’ Then click ‘Verify Java version’ and install.
  • If you are a Mac user, check that your computer has the latest Java installed (probably Java 6) and that it also is enabled. (Check it out at http://java.com or contact williams@cshl.edu for additional advice).
  • To test your Java installation, go to http://gfx.dnalc.org/files/apollo/test/ and follow the instructions at that site. The test is successful if an image opens up on your computer that looks like this screenshot: Java Screenshot

Make sure you have a valid iPlant account

Additional Readings and Assignments

  • ReviewDNA Subway manual (a printed copy will be presented at the workshop)
  • Review DNA Barcoding laboratory (a printed copy will be presented at the workshop)
  • Bring a small sample of plant tissue (a leaf/flower/etc.) or collect one on campus before the experiment.
  • If you wish to bring sequence data for the DNA barcoding or genome annotation experiments feel free to do so! 
  • Check out the slides and papers mentioned in the agenda.

Workshop Agenda and Resources

Thursday

Time

Topic

Slides

Notes/Links

09:00-09:15

Welcome/Logistics

 

 

09:15-10:00

Introductions

 

 

10:00-10:15

iPlant Collaborative Overview

Slides

iPlant Homepage
iPlant account portal

10:15-10:30

Overview, Workshop Objectives, DNA Subway Tour

Slides

DNA Subway Homepage

10:30-10:45

Break

 

 

10:45-11:15

Using DNA Subway to Teach Concepts in Molecular Biology

Slides

Gene Annotation Paper

11:15-12:00

Setting up projects for Annotation - Red Line

 

 

12:00-01:00

Lunch

 

 

01:00-01:15

DNA Barcoding setup

 

 

01:15-01:30

Mini-Seminar: DNA Barcoding

Slides

 

01:30-02:45

DNA Barcoding wet-lab cont'd

 

 

02:45-03:00

Mini-Seminar: Evidence Based Gene Modeling

 

 

03:15-04:45

Gene Annotation I

 

Annotation Files

04:45-05:00

Mini-Seminar: Alternative Splicing                                                      

Slides

Alternative Splicing Paper

Friday

Time

Topic

Slides

Notes/Links

09:00-09:30

Mini Seminar: Transposons                                                              

Slides

TARGeT Paper

09:30-10:15

Transposon Sleuthing: Yellow Line

 

 

10:15-10:30

Break

 

 

10:30-11:30

Gene Annotation II

 

ENCODE Project
ENCODE Nature

11:30-12:00

DNA Barcoding (PCR)

 

 

12:00-01:00

Lunch

 

 

01:30-02:30

DNA Barcoding - Blue Line


 

02:30-02:45

Break

 

 

02:45-03:45

Barcdoing electrophoresis and sequencing prep

 

 

03:45-04:30

Implementation and Classroom Planning

 

 

04:30-05:00

Summary, Evaluation, and Closure

 

 

Other Websites and Links and Resources

Attendees (23)

Name

Research Area

Email

Account Status

Mahnaz Kiani-Fariz

Genetics Nucleic Acids

mhkiani@tamu.edu

X

Jim Hu

Biochem Mol Struc Function

jim.hu.biobio@gmail.com

X

Idowu Famuyiwa

Training

idnoble_2@yahoo.com

X

Milton Daley

Biochem Mol Struct Function

mdaley@pvamu.edu

 

Ivonne Ruiz

Biochem Mol Struct Function

ivonne.garcia3@hccs.edu

X

Marie-therese Simms

Plant Biol

msimms@pvamu.edu

 

Karen Killion

Other

karen.killion@blinn.edu

X

Yi Lu

Algorithm Dev

yilu@pvamu.edu

X

Shaye Lewis

Dev Biol

sklewis@pvamu.edu

X

Damilola Olaleye

Cell Biol

dolaleye@pvamu.edu

X

Annette James

Agron

aajames@pvamu.edu

X

Joseph Oleniczak

Biotech/Molec Biol

jolenicz@austincc.edu

X

Vimlarani Chopra

Cell Biol

vimlarani.chopra@hccs.edu

 

Paul Johnson

Env Biol

pmjohnson@pvamu.edu

X

Paul Drummond

Mol Biosciences

pbdrummond@pvamu.edu

 

Deirdre Vaden

Genetics Nucleic Acids

dlvaden@pvamu.edu

 

Laura Carson

Chem

lecarson@pvamu.edu

 

Julia Stone

Cell Biol

jmstone@pvamu.edu

X

Shabnam Eslamfam

Computation Research

shabnam127@hotmail.com

X

Deforest Peterson

Cell Biol

df0pete@yahoo.com

X

Charity Woodard

Biochem Mol Struct Function

charity+woodard@yahoo.com

X

Quincy Moore

Cell Biol

qcmoore@pvamu.edu

X

Yoonsung Jung

Stats Probability

yojung@pvamu.edu

X

Post Workshop Survey

Please complete this survey at the conclusion of the workshop:

Post Workshop DNA Sequencing Results

Instructions on retrieving your results will be posted here after the workshop.

Gel Images   

DNA Subway instructions:

 

1.       Log into DNA Subway (dnasubway.iPlantcollaborative.org)

2.       Start a blue line project

3.       Under 'Select Project Type' select the project type to be ‘rbcL’ barcoding

4.       Under ‘Select Sequence Source’ choose ‘Import Sequence from DNALC’ Your sequences have a the tracking number: 10-240033358

and are posted as the entry under Jermel Watkins on 10/13/13

5.       Select the sequences you want to include and run your project; each person should have two sequences, 1 forward, 1 reverse.

           For example, Student 1 will have a sequence called Student1-M13F(21).... and a sample called Student1-M13R....

Important Pre-Workshop Travel Info

Recommended Lodging

There is no reimbursement for lodging. These hotels include those recommended by our hosting institution,but no guarantees are offered about the availability or suitability of the hotels.

Check website like hotels.com and travelocity.com for additional hotels and rates. 

America’s Best Value Inn & Suites

936-372-2227

19444 Stokes Road, Waller, TX 77484

www.bestvaluinnwaller.com

bestvaluewaller@yahoo.com

Parking Information/Directions:

http://www.pvamu.edu/pages/3911.asp

You will need a parking permit ($2) that can be obtained at the visitor's booth (entrance)

You should park at the Gravel Lot, ROTC lot or any open parking lot